File:Summary of RNA-Seq.svg

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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
Umhla
Imvelaphi Own work
Umbhali Thomas Shafee
Other versions

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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5 Eye Dwarha 2016

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Umhla/ixeshaThumbnailDimensionsUserKhawuphawule
Ngoku12:20, 13 EyoMdumba 2017Thumbnail for version as of 12:20, 13 EyoMdumba 20171 020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
12:19, 13 EyoMdumba 2017Thumbnail for version as of 12:19, 13 EyoMdumba 20171 020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 EyoMdumba 2017Thumbnail for version as of 10:48, 13 EyoMdumba 20171 020 × 803 (88 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
08:41, 10 EyoMdumba 2017Thumbnail for version as of 08:41, 10 EyoMdumba 20171 020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
05:08, 5 Eye Dwarha 2016Thumbnail for version as of 05:08, 5 Eye Dwarha 20161 020 × 803 (85 KB)Evolution and evolvabilityUser created page with UploadWizard

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